簡介 Q-Box AQUA采用間歇流動呼吸法,測量水生動物(脊椎動物和無脊椎動物均可)的代謝速率。即將水生動物放置在一個呼吸室中,通過溶解氧的減少來衡量代謝速率。耗氧率(VO2)通過軟件進行計算,原始溶解氧數(shù)據(jù)經(jīng)鹽度、溫度和壓力校正,計算結(jié)果顯示并保存在軟件中。 |
優(yōu)點 l 間歇流動呼吸法無需將動物從試驗箱中取出,測量依次進行,兩次測量之間用淡水沖洗試 驗箱,避免了傳統(tǒng) 密閉式測量方法導致的呼吸時內(nèi)產(chǎn)生缺氧狀態(tài)。 l 數(shù)據(jù)準確,讀數(shù)穩(wěn)定,維護簡單;· l 能長期地自動測量;· l 有不同大小呼吸室可以選擇,適宜于各種體積的水生動物測量 l 所有部件統(tǒng)一安裝在堅固的運輸箱中,便于攜帶和存放。 |
測量方式
在實驗過程中,水通過液流泵在動物室和帶光學溶解氧探針的流通容器中循環(huán)。由數(shù)字控制單元(DCU)控制三通閥,軟件控制設定系統(tǒng)的循環(huán)或沖洗模式。
在循環(huán)模式下進行呼吸測量,在沖洗模式下用水浴中的含氧水沖洗動物室。循環(huán)-沖洗-循環(huán),重復運行,直到實驗結(jié)束。沖洗和循環(huán)階段的時間由用戶在軟件中選擇。
用于小樣本的mini-AQUA
對于小型魚類和昆蟲幼蟲(~1g)的水生呼吸測量,Qubit提供了mini-AQUA套裝。改良版的Q-Box Aqua帶有小型水生呼吸室(9ml)和用于更小系統(tǒng)體積的組件。還包括一個微型室(體積為1.23ml),用于測量小于0.5g的幼蟲、卵和幼魚等非常小的樣品的耗氧率VO2。在測量期間,微型室采用間歇停止流動模式,并將溶解氧探針直接放置在該室中,從而提高系統(tǒng)的靈敏度和分辨率。
v 4個數(shù)據(jù)通道
v 可變的數(shù)據(jù)采集速率
v 圖表、表格和儀表顯示
v 分析功能套件:
(統(tǒng)計、曲線擬合、積分等)
v 可輸入數(shù)據(jù)控制設備。
v 可生成多頁報告
v 數(shù)據(jù)回放功能
v 可輸入和編輯簡單公式
v 自動多種數(shù)學函數(shù)
v 動態(tài)自動計算
應用領域
v 水生生物呼吸作用研究(測試溫度、缺氧、養(yǎng)分、壓力、體型對呼吸作用影響);
v 脊椎動物和無脊椎動物研究
v 實驗室或野外均可應用
基本組件
v 光學DO探頭和水浴
v 小型呼吸測量室(140ml)
v 大呼吸測量室(660ml)
v 水浴用溫鹽探頭
v 液體泵(1 LPM)和三通閥
v 三通閥數(shù)字控制單元
v 4通道數(shù)據(jù)接口
v 數(shù)據(jù)采集軟件
v 絕對壓力傳感器
v 堅固的防風雨外殼
v 可選電池組
技術(shù)參數(shù)
客戶評價
“We have been using the Q-Box AQUA respirometry package for a year and have been impressed with the quality and versatility of the system. Although designed for teaching, we have found it to be more than adequate for basic research applications on both fish and cephalopods. The mechanical systems are well designed and easily serviced and the software is intuitive and flexible enough for use in diverse applications. The technicians at Qubit are outstanding; they were able to modify the system to suit our particular needs and they have always been quick to respond when technical support was required. The fact that Qubit ships the system in a protective case was also a big plus for field work!”
form Dr. Tyson MacCormak, Biology Department, Mount Alison University, New Brunswick, Canada
“我們已經(jīng)使用Q-Box AQUA呼吸測量軟件包一年了,它的質(zhì)量和多功能性給我們留下了深刻的印象。雖然是為教學而設計的,但我們發(fā)現(xiàn)它對于魚類和頭足類的基礎研究應用來說已經(jīng)足夠了。機械系統(tǒng)設計精良,維修方便,軟件直觀靈活,適用于多種應用。Qubit的技術(shù)人員非常出色;他們能夠修改系統(tǒng)以滿足我們的特殊需要,且技術(shù)支持響應迅速。Qubit系統(tǒng)裝在一個保護箱里,非常有益于野外工作!
發(fā)表文獻
· Bessemer RA et al. (2015) Cardiorespiratory toxicity of environmentally relevant zinc oxide nanoparticles in the freshwater fish Catostomus commersonii. Nanotoxicology 9: 861-870
· Callaghan NI et al. (2016) Zinc oxide nanoparticles trigger cardiorespiratory stress and reduce aerobic scope in the white sucker, Catostomus commersonii. Nanoimpact 2:29-37
· Black MN et al. (2017) Environmentally relevant concentrations of amine-functionalized copper nanoparticles exhibit different mechanisms of bioactivity in Fundulus Heteroclitus in fresh and brackish water. Nanotoxicology 11:1070-1085
· Keogh C et al. (2017) The double edge to parasite escape: invasive host is less infected but more infectable. Ecology 98:2241-2247
· Rangel RE, Johnson DW (2018) Metabolic responses to temperature in a sedentary reef fish, the bluebanded goby (Lythrypnus dalli, Gilbert). Journal of Experimental Marine Biology and Ecology 501: 83-89
Lamarre SG et al. (2019) Interrelationship Between Contractility, Protein Synthesis and Metabolism in Mantle of Juvenile Cuttlefish (Sepia officinalis). Frontiers in Physiology V10, 1-14 (doi: 10.3389/fphys.2019.01051)
產(chǎn)地:加拿大